Direkt zum InhaltDirekt zur SucheDirekt zur Navigation
▼ Zielgruppen ▼

Humboldt-Universität zu Berlin - IRI Life Sciences

Humboldt-Universität zu Berlin | IRI Life Sciences | News | Microtubules are fun - manual dataanalysis not: New paper online from Simone Reber

Microtubules are fun - manual dataanalysis not: New paper online from Simone Reber

Great collaboration between IRI Life Sciences and BIMSB: Together with colleagues Junior Group Leader Dr. Simone Reber published her manuscript on bioRxiv.
MTrack: Automated Detection, Tracking, and Analysis of Dynamic Microtubules

Abstract

Microtubules are polar, dynamic filaments fundamental to many cellular processes. In vitro reconstitution approaches with purified tubulin are essential to elucidate different aspects of microtubule behavior. To date, deriving data from fluorescence microscopy images by manually creating and analyzing kymographs is still commonplace. Here, we present MTrack, implemented as a plug-in for the open-source platform Fiji, which automatically identifies and tracks dynamic microtubules with sub-pixel resolution using advanced objection recognition. MTrack provides automatic data interpretation yielding relevant parameters of microtubule dynamic instability together with population statistics. The application of our software produces unbiased and comparable quantitative datasets in a fully automated fashion. This helps the experimentalist to achieve higher reproducibility at higher throughput on a user-friendly platform. We use simulated data and real data to benchmark our algorithm and show that it reliably detects, tracks, and analyzes dynamic microtubules and achieves sub-pixel precision even at low signal-to-noise ratios.

 

 

The article can be accessed https://www.biorxiv.org/content/early/2018/07/13/368191