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Humboldt-Universität zu Berlin | IRI Life Sciences | Events | Colloquium | Cell Communication | IRI-Schering Colloquium - Amy Buck: "RNA and vesicle secretion by helminth parasites: at the host interface"

IRI-Schering Colloquium - Amy Buck: "RNA and vesicle secretion by helminth parasites: at the host interface"

University of Edinburgh
What
  • Cell Communication
  • Colloquium
When Apr 05, 2018 from 04:00 PM to 05:30 PM (Europe/Vienna / UTC200) iCal
Where IRI Life Sciences, Philippstr. 13, Bld. 18, 3rd Floor
Contact Name

coll.pngSchering

 

 

If you like to meet our speaker for a personal discission, please contact stefanie.scharf@iri-lifesciences.de

 

Abstract: RNA interference pathways underpin defence and adaptation strategies in nematodes, but their roles in parasitism are largely unexplored. We previously showed that the gastrointestinal nematode Heligmosomoides polygyrus bakeri (Hpb) exports small RNAs in extracellular vesicles that are internalized by mouse cells, and these suppress host immune responses in vitro and in vivo. We further showed that raising antibodies against the EVs confers protection to infection, suggesting EVs are an important mechanism of host modulation. Here we report the full spectrum of small RNAs in these vesicles, based on a new assembly and annotation of the Hpb genome. The dominant class of RNAs in EVs is siRNAs generated by RNA-dependent RNA polymerases inside the nematode and there is a specific enrichment in siRNAs derived from novel repetitive and transposable elements. Small RNA sequencing of mouse epithelial cells following EV treatment suggests a selective subset of the nematode siRNAs and miRNAs are present at functionally relevant concentrations and may interact with host genes with high degrees of complementarity. We further characterize one specific vesicular Argonaute protein that is highly conserved in the parasitic species but rapidly diverged in free-living Caenorhabditis and confirm its presence in other parasitic strongyle extracellular products. Our findings point to selectivity in the loading of newly evolved siRNAs in EVs from nematode parasites and suggest a novel Argonaute protein may mediate this.