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Humboldt-Universität zu Berlin - IRI Life Sciences

Humboldt-Universität zu Berlin | IRI Life Sciences | Events | Colloquium | Series: Life in Numbers | IRI-Schering-Colloquium - Johan Elf: “Probing intracellular kinetics at single molecule sensitivity”

IRI-Schering-Colloquium - Johan Elf: “Probing intracellular kinetics at single molecule sensitivity”

Uppsala University
When Sep 07, 2017 from 04:00 PM to 05:30 PM (Europe/Vienna / UTC200) iCal
Where IRI Life Sciences, Philippstr. 13, Bld. 18, 3rd Floor
Contact Name

 

coll.png​   Schering

 

 

Johan Elf is Professor in Biological Physicsat the Uppsala University.

The overall ambition of the Elf Lab is to bridge the gap between quantitative physical models and biological observations in order to identify and resolve inconsistencies in our current understanding of life at the molecular level.

 

Abstract:

Cas9-based DNA targeting is radically different from that of transcription factors. Cas9 can be programmed by a guide RNA to target any sequence, at the price of the increased time required to open cellular dsDNA to test for complementarity. We have studied how much time it takes to located a singel target sequence in a living cell using single molecule fluorescence microscopy. We also measure how long time the bound dCas9 stays at a target sequences and what implications this has for repression of a targeted gene.

 

If you like to meet our speaker for a personal discission, please contact stefanie.scharf@iri-lifesciences.de