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Humboldt-Universität zu Berlin | IRI Life Sciences | Events | IRI Talks | Dates | IRI-Talk - Carsten Wolff: "Multi-view light-sheet imaging and cell tracking reveal the cellular basis of limb morphogenesis"

IRI-Talk - Carsten Wolff: "Multi-view light-sheet imaging and cell tracking reveal the cellular basis of limb morphogenesis"

What
  • IRI Talk
When Jan 21, 2019 from 04:00 PM to 05:00 PM (Europe/Vienna / UTC100) iCal
Where IRI Life Sciences, Humboldt-Universität zu Berlin, Philippstr. 13, Michaelis Building (No 18), Maud Menten Hall (3rd Floor)
Contact Name

Invitation lila

Dr. Carsten Wollf (Humboldt-Universität zu Berlin)

Abstract:

Multi-view light-sheet imaging and cell tracking reveal the cellular basis of limb morphogenesis

During development coordinated cell behaviors orchestrate tissue and organ morphogenesis to suit the lifestyle of the organism. We have used here the crustacean Parhyale hawaiensis to study the cellular basis of limb development. Transgenic Parhyale embryos with fluorescently labeled nuclei were imaged at high spatiotemporal resolution using multi-view light-sheet fluorescence microscopy over several days of embryogenesis spanning appendage morphogenesis from early specification up to late differentiation stages.

To be able to analyze the terabyte-sized data sets we used a new tool called Massive Multi-view Tracker (MaMuT) for cell tracking that enabled us to reconstruct the complete cell lineage of an outgrowing limb with single-cell resolution.

The quantitative analyses about cell behaviors show that the limb primordium in Parhyale becomes subdivided from an early stage into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. These findings validate the boundary model originally proposed by Hans Meinhardt. This model postulates that a secondary developmental field, i.e. the proximodistal axis of a limb, is specified during embryogenesis de novo relative to the main anteroposterior and dorsoventral body axes.