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Humboldt-Universität zu Berlin - IRI Life Sciences

Humboldt-Universität zu Berlin | IRI Life Sciences | Events | Tutorials | Past events | Tutorial in Life Sciences - Daniel Müller: "Atomic Force Microscopy"

Tutorial in Life Sciences - Daniel Müller: "Atomic Force Microscopy"

  • Past
  • Tutorial
When Apr 24, 2015 from 09:00 AM to 12:00 PM (Europe/Vienna / UTC200) iCal
Where Philippstr. 13 Building 18, Maud Menten Hall

Daniel Müller

ETH Zurich


Atomic force microscopy as a multifunctional molecular toolbox to characterize biological systems

With its ability to observe, manipulate and explore the functional components of the biological cell at subnanometre resolution, atomic force microscopy (AFM) has produced a wealth of new opportunities

in nanobiotechnology. Evolving from an imaging technique to a multifunctional ‚lab-on-a-tip‘, AFM based force spectroscopy is increasingly used to study the mechanisms of molecular recognition and protein folding, and to probe the local elasticity, chemical groups and dynamics of receptor–ligand interactions in live cells. AFM cantilever arrays allow the detection of bioanalytes with picomolar sensitivity, opening new avenues for medical diagnostics and environmental monitoring. Here I will review the fascinating opportunities offered by

the rapid advances in AFM.


Force probing surfaces of living cells to molecular resolution

Biological processes rely on molecular interactions that can be directly measured using force spectroscopy techniques. Here I will describe how atomic force microscopy can be applied to force probe surfaces of living cells to single-molecule resolution. Such probing of individual interactions can be used to map cell surface receptors, and to assay the receptors‘ functional states, binding kinetics and landscapes. This information provides unique insight into how cells structurally and functionally modulate the molecules of their surfaces to interact with the cellular environment.